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ChemiSCREEN GPR14 Membrane Preparation

品牌
Sigma-Aldrich
货号
HTS033M
包装型号
规格纯度
价格
10463.8 *本价格含增值税费
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半岛bd体育手机客户端 名称:
ChemiSCREEN GPR14 Membrane Preparation
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

TRANSFECTION: Full-length human GPR14 cDNA
Urotensin II, a cyclic 11-13 residue peptide expressed in motoneurons of the spinal cord, acts as a potent vasoconstrictor (Coulouarn et al., 1998; Ames et al., 1999). The effects of urotensin II are mediated by binding to a GPCR, GPR14, which is expressed in endothelium, smooth muscle, heart and pancreas (Ames et al., 1999). Genetic deletion of GPR14 in mice renders aortae refractile to the contractile activity of urotensin II without changing baseline hemodynamics (Behm et al., 2003). Pharmacological inhibition of urotensin II/GPR14 interactions prevents renal insufficiency following renal artery ligation (Clozel et al., 2004). Thus, GPR14 is an attractive target for a number of cardiovascular diseases. Chemicon′s GPR14 Membrane Preparations are ideal tools for screening for antagonists of urotensin II/GPR14 interactions. The membrane preparations exhibit a Kd of 0.4 nM for [125I]-urotensin II. With 10 mg/well GPR14 Membrane Prep and 0.3 nM [125I]-urotensin II, a greater than 20-fold signal-to-background ratio is obtained.

应用

Radioligand binding assay.
Human UT / UT1 / GPR14 GPCR membrane preparation for Radioligand binding Assays.

生化/生理作用

  • GPCR Class: A
  • Protein Target: UT/ UT1/ GPR14
  • Target Sub-Family: Urotensin II

半岛bd体育手机客户端 规格

Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

外形

Membranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80℃. One vial typically contains 2000 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific

储存及稳定性

Maintain frozen at -70℃ for up to 2 years. Do not freeze and thaw.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

基本信息

eCl@ss 32161000

半岛bd体育手机客户端 性质

质量水平 100
生物来源 human
重组 expressed in Chem-2 cells
manufacturer/tradename ChemiScreen
Chemicon?
technique(s) radioligand binding assay (RLBA): suitable
NCBI登记号 NM_018949.1
UniProt登记号 Q9UKP6

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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