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ChemiScreen CXCR2 Membrane Preparation

品牌
Sigma-Aldrich
货号
HTS002M
规格纯度
Human CXCR2 / IL-8b GPCR membrane preparation for Radioligand binding Assays.
价格
10463.8 *本价格含增值税费
促销
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半岛bd体育手机客户端 名称:
ChemiScreen CXCR2 Membrane Preparation
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

CXCR2 is a 7-TM G-protein coupled receptor that binds to the chemokines GROalpha, GRObeta, GROgamma, IL-8, ENA-78, NAP-2 and GCP-2 (Olson and Ley, 2002). Neutrophils, mast cells and microvascular endothelial cells express CXCR2, and interactions of CXCR2 with its ligands promotes chemotaxis of these cell types (Heidemann et al., 2003; Nilsson et al., 1999; White et al., 1998). Studies with mice lacking CXCR2 indicate that CXCR2 promotes growth of primary tumors and secondary metastases (Keane et al., 2004), and plays an essential role in hyperoxia-induced lung injury (Sue et al., 2004). In addition, cytomegalovirus encodes a CXCR2-binding chemokine, vCXC-1, that promotes neutrophil migration to infected cells (Penfold et al., 1999). Chemicon′s CXCR2 Membrane Preps are ideal tools for screening for antagonists of interactions between CXCR2 and its ligands. Radiolabeled IL-8 binds to the CXCR2 membranes with a Kd of 0.075 nM. With 0.1 nM 125I-labeled IL-8, a 5-fold window of specific binding is obtained.
TRANSFECTION: Full-length human CXCR2 cDNA (Accession Number: M73969)

应用

Radioligand binding assay.

生化/生理作用

Target Sub-Family: Chemokine
GPCR Class: A
Protein Target: CXCR2 / IL-8b

质量

Bmax for [125I]IL-8 binding: 0.93 pmol/mg protein; Kd for [125I] IL-8 binding: ~ 0.075 nM

外形

Membranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial typically contains 1000 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of 125I-labeled IL-8 (0.1 nM input concentration).

储存及稳定性

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32161000
NACRES NA.84

半岛bd体育手机客户端 性质

质量水平 100
生物来源 human
重组 expressed in Chem-1 cells
manufacturer/tradename ChemiScreen
Chemicon?
浓度 0.5 mg/mL
technique(s) radioligand binding assay (RLBA): suitable
NCBI登记号 NM_001557.2
UniProt登记号 P25025
运输 dry ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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