CF^TM488A maleimide

半岛bd体育手机客户端 说明

一般描述

CF^?488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line. Under common detection conditions, CF^?488A is at least as bright as Alexa Fluor^?488. However, a major advantage of CF^?488A over Alexa Fluor^?488 is that antibody conjugates prepared from the former are biologically more specific. Alexa Fluor^?488 carries multiple negative charges, which can significantly change the isoelectric point of the proteins the dye labels and consequently alter the specificity of the protein conjugates. CF^?488A, on the other hand, is minimally charged. Thus, antibody conjugates prepared from the dye ensure biological detection with high signal-to-noise ratio. Another feature of CF^?488A is that the emission peak wavelength is about 10 nm shorter than that of Alexa Fluor^?488 and 15 nm shorter than that of the traditional green dye FITC (or FAM). The shorter wavelength of CF^?488A offers the advantage of less fluorescence "spill-over" in the red channel in multi-color detection applications.

应用

CF^?488A maleimide has been used to tag FtsN (cell division protein), a protein involved in bacterial cell division machinery.

特点和优势

1. Yields biologically more specific antibody conjugates and has less "spill-over" fluorescence in the red channel than Alexa Fluor^?488. 2. Extremely photostable. 3. Highly water-soluble and pH-insensitive.

制备说明

Protect from light

重悬

Dissolve in DMSO

法律信息

This product is distributed by sigma-aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a registered trademark of Life Technologies
CF is a trademark of Biotium, Inc.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

质量水平	100
测定	≥90% (TLC)
形式	solid
分子量	~1036 g/mol
溶解性	DMF: soluble DMSO: soluble H2O: soluble
ε (extinction coefficient)	70000 at 490 nm
吸光度比值	A280/490 nm0.1
荧光	λex488 nm (Flow cytometry laser line) λex488 nm (Microscopy laser line) λex490 nm; λem515 nm
适用性	suitable for fluorescence
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable