您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> CD45 Active human

CD45 Active human

品牌
Sigma-Aldrich
货号
SRP0219
规格纯度
recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)
价格
5691.76 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
CD45 Active human
CD45R
T200 leukocyte common antigen
Leukocyte common antigen (LCA)
PTPRC
Protein tyrosine phosphatase, receptor type, C
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 is expressed on T-cells and is found in multiple isoforms. The extracellular domain of the protein is predicted to have an amino-terminal mucin-like segment, a cysteine-rich domain and three fibronectin type 3 domains. The gene encoding this protein is localized on human chromosome 1q31.3.
Protein tyrosine phosphatase. Catalytic domain, (584-1256) (Gene bank accession number NM_080922) with N-terminal His tag, MW = 75 kDa, expressed in a Baculovirus Sf9 expression system.

应用

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

生化/生理作用

Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 acts as a protein tyrosine phosphatase in leucocyte signaling. It has a role in flow cytometric diagnostics in leukemia. By dephosphorylation of Src kinases, the protein acts as a regulator of antigen receptor signaling in T and B cells. As a negative regulator of Janus kinases, CD45 inhibits cytokine receptor signaling.

单位定义

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃.

外形

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRES NA.32

半岛bd体育手机客户端 性质

生物来源 human
重组 expressed in baculovirus infected insect cells
测定 ≥90% (SDS-PAGE)
形式 aqueous solution
分子量 75 kDa
包装 pkg of 20 μg
储存条件 avoid repeated freeze/thaw cycles
浓度 >0.02 mg/mL
NCBI登记号 NM_080922
UniProt登记号 P08575
运输 dry ice
储存温度 ?70℃
Gene Information human ... PTPRC(5788)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map