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Caspase 8 Colorimetric Activity Assay Kit, IETD

品牌
Sigma-Aldrich
货号
APT171
规格纯度
Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.
价格
9197.07 *本价格含增值税费
促销
服务
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  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Caspase 8 Colorimetric Activity Assay Kit, IETD
FLICE
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.

应用

Research Category
Apoptosis & Cancer
Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.

组分

5X Cell Lysis Buffer (Part No. 90065): 5 mL

5X Assay Buffer (Part No. 90066): 10 mL

Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90082): 1 mL of 2.5 mg/mL solution

Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 μM (0.05 mg/mL) in DMSO

pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO

储存及稳定性

Store kit materials at -20°C up to their expiration date.

Special Precautions:

· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.

· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32161000

半岛bd体育手机客户端 性质

质量水平 100
species reactivity (predicted by homology) mammals
manufacturer/tradename Chemicon?
technique(s) activity assay: suitable
NCBI登记号 NM_001080124.1
NM_001080125.1
NM_001228.4
NM_033355.3
NM_033356.3
NM_033358.3
UniProt登记号 Q14790
detection method colorimetric
运输 dry ice

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2

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