Carbonic Anhydrase 2/CA2 human

半岛bd体育手机客户端 说明

一般描述

Carbonic Anhydrase 2 (CA2) is a zinc metalloenzyme and it helps in regulating acid-base balance in cells. It is a ubiquitous cytosolic protein. Carbonic anhydrase 2 also known as carbonate dehydratase II, carbonic anhydrase C, belongs to the class of a carbonic anhydrases. The associated gene is mapped to human chromosome 8q21.

应用

Carbonic Anhydrase 2/CA2 human has been used for CA2 ELISA (enzyme-linked immunosorbent assay).

生化/生理作用

Carbonic Anhydrase 2 (CA2) catalyzes the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa). CA2 can bind to various transporters, such as anion exchanger 1 (AE1), NBCe1 (Na(+)/HCO3(-) cotransporter), NHE3 (Na(+)/H(+) exchanger 3) and MCT (monocarboxylate transporter). This association increases the rate of transport. Mutations in the CA2 gene are responsible for carbonic anhydrase II (CAII) deficiency syndrome, an autosomal recessive osteopetrosis characterised with renal tubular acidosis as well as cerebral calcifications. Changes in the expression of CA2 are linked with carcinogenesis.

外形

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT. Normally Mannitol or Trehalose is added as protectants before lyophilization.

重悬

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 month. For extended storage, it is recommended to store at -20℃.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed in HEK 293 cells
标签	6-His tagged (C-terminus)
测定	≥95% (SDS-PAGE)
形式	lyophilized
分子量	calculated mol wt 30 kDa observed mol wt 30 kDa (DTT-reduced. Ser 2 is the predicted N-terminal .)
包装	pkg of 10 μg pkg of 50 μg
杂质	<1 EU/μg endotoxin (LAL test)
UniProt登记号	P00918
运输	wet ice
储存温度	?20℃
Gene Information	human ... CA2(760)

安全信息

象形图
警示用语：	Warning
危险声明	H319
预防措施声明	P264 - P280 - P305 + P351 + P338 - P337 + P313
危险分类	Eye Irrit. 2
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable