BioTracker LYSO-TP Live Cell Dye

半岛bd体育手机客户端 说明

一般描述

Hypochlorous acid (HOCl) is a highly potent reactive oxygen species (ROS) and helps eliminate pathogens in the innate immune system. Mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. Subcellular detection of HOCL is currently limited due to low concentration, strong oxidization, and short lifespan of the analyte.

The BioTracker LYSO-TP dye is a live cell two-photon green fluorescent turn-on imaging probe for HOCL. The probe targets HOCL specifically in the lysosomes of cells. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical push?pull (amineketone) structure. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement. To monitor subcellular HOCL levels, morpholine was introduced at a long distance from the reaction site to ensure the probes intracellular localization.

Spectral Properties
Absorbance: 375nm
Emission: 500nm

应用

Research Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging

质量

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 357.49 g/mol

外形

Lyophilized

储存及稳定性

Store BioTracker LYSO-TP Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

测定	≥98% (H-NMR) ≥98% (HPLC) ≥98% (LC/MS) ≥98% (elemental analysis)
technique(s)	cell based assay: suitable
detection method	fluorometric
运输	ambient

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable