AOF ITS Supplement (100X), 100ml | SCM055

半岛bd体育手机客户端 说明

一般描述

EMD Millipore’s AOF ITS (100X) supplement is an animal-origin-free product that contains human recombinant insulin, human recombinant transferrin along with sodium selenite.

Undefined animal derived media supplements, such as fetal bovine serum, have recently been an area of concern for many cell culturists due to their increased costs, lot to lot variability and increased regulation worldwide. The elimination of fetal bovine serum from cell culture media provides a more defined enviroment to study basic biological questions. Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies such as insulin, transferrin and selenite.

ITS supplements have been proven to replace serum and grow the following cells in serum free or reduced serum conditions: CHO, HEK293, fibroblasts, hybridoma, VERO cells, Hela, HT29, MDCK, Mesenchymal, IPS Cells, Cardiac, kidney, keratinocyte, embryonic, neural stem cell, hepatocytes.

应用

Research Category
Stem Cell Research
EMD Millipore’s AOF ITS (100X) supplement is an completely animal-origin-free product

组分

All components are derived using AOF (Animal-Origin-Free) reagents and protocols.

Recombinant Human Insulin is derived from Pichia pastoris (yeast).
Recombinant Human Transferrin is derived from Oryza sativa (rice).
Sodium Selenite is a synthetic chemical.

质量

Tested for the ability to support the proliferation of VERO cells in serum free conditions over a 3 day time period.
pH: 7.6-8.0.
Product is sterile.
Endotoxin levels are ≤ 1 EU/mg.

外形

Product is made in an Earle′s Balanced Salt Solution and filtered through a 0.22 micron membrane before vialing. Solution is a light pink clear solution that is free of particulates.

储存及稳定性

Store at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.

分析说明

Control
VERO cells

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160801
NACRES	NA.75

半岛bd体育手机客户端 性质

质量水平	100
形式	liquid
浓度	100 ×
technique(s)	cell culture | mammalian: suitable cell culture | stem cell: suitable

安全信息

象形图
警示用语：	Warning
危险声明	H302
预防措施声明	P264 - P270 - P301 + P312 - P501
危险分类	Acute Tox. 4 Oral
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable