Anti-ZFX Antibody, clone 3E8.1

半岛bd体育手机客户端 说明

一般描述

ZFX, also known as Zinc finger X-chromosomal protein, and encoded by the gene name ZFX, is probable transcriptional activator. This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined.

免疫原

GST-tagged recombinant protein corresponding to human ZFX.

应用

Immunocytochemistry Analysis: 2.0 ?g/mL from a representative lot detected ZFX HeLa cells.
Research Sub Category
Nuclear Receptors
This Anti-ZFX antibody is validated for use in WB, IC for the detection of ZFX.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected ZFX in 200 ?g of HeLa nuclear extract.

目标描述

~130, ~90, ~59 kDa observed. Three bands (~130 kDa, ~91 kDa and ~59 kDa) corresponding to ZFX were detected. The ~130 kDa band is nuclear, while the ~91 kDa band was found in the cytoplasmic (Zhu, Z., et al. (2013). J Neurooncol. 112:17-25).

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	3E8.1, monoclonal
species reactivity	human
technique(s)	immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_001171555
UniProt登记号	P17010
运输	wet ice
Gene Information	human ... ZFX(7543)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2