Anti-WNT10B Antibody, clone 5A7

半岛bd体育手机客户端 说明

一般描述

Wingless-type MMTV integration site family member 10B (WNT10B), also known as Protein Wnt-12 (WNT12), consists of structurally related secreted signaling proteins implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is detected in most adult tissues, with the highest levels found in heart and skeletal muscle, and lower levels found in brain. WNT10B may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. Defects in WNT10B are linked to split-hand/foot malformation type 6 (SHFM6).

免疫原

Purified recombinant fragment of human WNT10B expressed in E. coli.

应用

Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-WNT10B Antibody, clone 5A7 is a highly specific mouse monoclonal antibody, that targets Wnt & has been tested in IHC, western blotting & Immunofluorescence.
Immunohistochemistry Analysis: A 1:500-1,000 dilution from a representative lot detected WNT10B in human kidney and liver tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT10B in PANC-1 cells.

Optimal working dilutions must be determined by end user.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WNT10B in 10 ?g of HeLa cell lysate.

目标描述

~47 kDa observed. Uncharacterized bands may appear in some lysate(s).

外形

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	5A7, monoclonal
species reactivity	human
technique(s)	immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NP_003385
UniProt登记号	O00744
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable