Anti-WIPI-2, clone 1B8.1 Antibody

半岛bd体育手机客户端 说明

一般描述

WIPI-2, also known as Atg18, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.

免疫原

GST-tagged recombinant protein corresponding to the C-terminus of human WIPI-2.
Epitope: C-terminus

应用

Research Sub Category
Apoptosis - Additional
This Anti-WIPI-2, clone 1B8.1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of WIPI-2.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected WIPI-2 in human pancreas tissue.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected WIPI-2 in 10 ?g of human testis tissue lysate.

目标描述

~49 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	1B8.1, monoclonal
species reactivity	human
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_001028690
UniProt登记号	Q9Y4P8
运输	wet ice
Gene Information	human ... WIPI2(26100)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable