Anti-WIPI-2 antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

The WIPI2 gene is mapped on the human chromosome at 7p22.1. WIPI2 has a 7-bladed propeller structure and contains a conserved motif.
WD repeat domain, phosphoinositide-interacting 2 (WIPI-2) belongs to the WIPI protein family. It is localized on the autophagy membrane and plasma membrane. Apart from this, it is also present on the membranes near the Golgi cisternae. WIPI2 is a mammalian ortholog of Atg18, which is ubiquitously expressed in a variety of cell lines.

特异性

Anti-WIPI-2 recognizes human, rat, and mouse WIPI-2.

应用

Anti-WIPI-2 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

生化/生理作用

WIPI-2 (WD repeat domain, phosphoinositide-interacting 2) plays a vital role in the transformation of omegasomes into autophagosomes. The above protein functions as a mammalian effector of phosphatidylinositol 3-phosphate (PtdIns3P). WIPI2 aids recombinant capsid protein VP1 (rVP1) in upregulating autophagy, MAPK1 (Mitogen-activated protein kinase 1)/3 phosphorylation and matrix metallopeptidase 9 (MMP-9) activity, which are required for macrophage migration.

外形

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2–8 ℃ for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen ~49 kDa
species reactivity	human, mouse, rat
浓度	~1.0 mg/mL
technique(s)	immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells. western blot: 1-2 μg/mL using using whole extracts of HEK-293T cells over-expressing mouse WIPI-2.
UniProt登记号	Q9Y4P8
运输	dry ice
储存温度	?20℃
Gene Information	human ... WIPI2(26100) mouse ... Wipi2(74781) rat ... Wipi2(288498)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable