半岛bd体育手机客户端 说明
一般描述
WAC (WW domain-containing adapter protein with coiled-coil) functions as a linker between gene transcription and histone H2B monoubiquitination at ′Lys-120′ (H2BK120ub1). Wac interacts with the RNA polymerase II transcriptional machinery through its WW domain and with RNF20-RNF40 via its coiled-coil region. It has been shown to regulate H2BK120ub1, gene transcription, cell-cycle checkpoint activation in response to DNA damage, positively regulate amino acid starvation-induced autophagy and negatively regulate the ubiquitin proteasome pathway. In nuclear speckles it has been observed to colocalize with pre-mRNA processing complexes. This antibody recognizes the N-terminus of the WAC protein.
免疫原
Recombinant protein corresponding to human WAC.
应用
Western Blotting Analysis: 1 ?g/mL from a representative lot detected WAC in 10 ?g of HEK293 cell lysate.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected WAC, N-terminus in 293T cell lysates stably transfected with SFB-RNF20 and in HCT116 cell lysates transfected with siRNA negative control (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected WAC, N-terminus in HCT116 cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in HCT166 cell lysates transfected with siRNF20 (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
Anti-WAC, N-terminus detects levels of WAC proteins & has been published & validated for use in WB, IP & ChIP.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
质量
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 1 ?g/mL of this antibody detected WAC, N-terminus in 10 ?g of K562 cell lysate.
目标描述
~80 kDa observed. Multiple isoforms are known to exist at ~70 kDa, ~65 kDa, ~60 kDa, and ~47 kDa Uncharacterized bands may be observed at ~52 kDa, ~75 kDa, and ~270 kDa in some cell lysates.
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
K562 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | mouse, human |
| technique(s) | ChIP: suitable immunoprecipitation (IP): suitable western blot: suitable |
| NCBI登记号 | NP_057712 |
| UniProt登记号 | Q9BTA9 |
| 运输 | wet ice |
| Gene Information | human ... WAC(51322) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
