半岛bd体育手机客户端 说明
一般描述
VAC14 gene is mapped to human chromosome 16q22.1-q22.2. VAC14 is a scaffold protein and is a component of the PIKfyve protein kinase complex.
特异性
Anti-VAC14 (C-terminal) specifically recognizes human, mouse, and rat VAC14.
应用
Anti-VAC14 (C-terminal) antibody produced in rabbit has been used in:
生化/生理作用
VAC14 phosphorylates PtdIns(3)P to generate the housekeeping phospholipid PtdIns(3,5)P2. It associates with and upregulate PIKfyve phosphoinositide-5-kinase activity. VAC14-PIP5K3- PtdIns(3,5)P2pathway is thought to be physiologically linked to insulin activation of glucose transport into the cell. Knockout of the VAC14 gene in mice results in massive neurodegeneration and particularly affected are neurons in the midbrain and of the peripheral sensory system.
VAC14 is a regulator of PIP5K3/PIKfyve, a dual specificity kinase.
Mutation in VAC14 gene leads to the development of retinitis pigmentosa (RP).
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
Store at –20 ℃. For continuous use, the product may be stored at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots at –20 ℃. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~82 kDa |
| species reactivity | rat, mouse, human |
| 包装 | antibody small pack of 25 μL |
| 浓度 | ~1.5 mg/mL |
| technique(s) | immunoprecipitation (IP): 10-15 μg using A431 cell lysates indirect immunofluorescence: 8-16 μg/mL using HeLa cells western blot: 1-2 μg/mL using extracts of mouse brain (S1 fraction) or rat brain (S1 fraction) |
| UniProt登记号 | Q08AM6 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... VAC14(55697) rat ... Vac14(307842) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
