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位置: 首页> 品牌> Sigma-Aldrich> Anti-unmodified-Histone H3 (Lys9) antibody, Mouse monoclonal

Anti-unmodified-Histone H3 (Lys9) antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200591
规格纯度
clone 9B1-2G6, purified from hybridoma cell culture
价格
5045.46 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-unmodified-Histone H3 (Lys9) antibody, Mouse monoclonal
Monoclonal Anti-unmodified-Histone H3 (Lys 9) antibody produced in mouse
Anti-HIST1H3E H3FD
Anti-HIST1H3C H3FC
Anti-HIST1H3J H3FJ
Anti-HIST1H3H H3FK
Anti-HIST1H3B H3FL
Anti-HIST2H3D
Anti-HIST1H3A H3FA
Anti-HIST1H3I H3FF
Anti-HIST2H3A
Anti-HIST1H3D H3FB
Anti-HIST1H3F H3FI
Anti-HIST2H3C H3F2 H3FM
Anti-HIST1H3G H3FH
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-unmodified-Histone H3 (Lys9 ) (mouse IgG1 isotype) is derived from the hybridoma 9B1-2G6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. Histone H3 is a core component of histone octamer of nucleosome along with other histones H4, H2A and H2B. Histone H3 comprises lysine and arginine residues in the N-terminal region. Conserved lysine residues in the N-terminal tail domains of histone H3 comprise theLys4, Lys9 and Lys27, which are the preferred sites of methylation.

特异性

Monoclonal Anti-unmodified-Histone H3 (Lys9 ) recognizes human unmodified-Histone H3 (Lys9 ) (nontested in other species). This antibody also binds to peptides containing mono-, and di-methyl-Lys4 and acetyl-Lys14 of Histone H3. It does not bind to peptides containing mono-, di-, and tri-methyl-Lys9 and acetylLys9 of Histone H3.

免疫原

Peptide corresponding to amino acids surrounding Lys9of human Histone H3, conjugated to KLH.

应用

Monoclonal Anti-unmodified-Histone H3 (Lys9) antibody produced in mouse has been used in:

  • immunostaining
  • immunoblotting
  • immunofluorescence

生化/生理作用

Histones are subjected to extensive covalent modifications, including phosphorylation, methylation, acetylation and ubiquitination. These modifications are thought to play an important role in development and cancer. Histone H3 methylation at Lys9 is a modification intrinsically linked to epigenetic silencing and heterochromatin assembly. Histone methylation, as like acetylation, is a complex, dynamic process involving several processes, including transcriptional regulation, chromatin condensation, mitosis, and heterochromatin assembly. Moreover, lysine residues can be mono-, di-, and tri-methylated, adding further complexity to the regulation of chromatin structure.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage,freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
抗体形式 purified from hybridoma cell culture
antibody product type primary antibodies
克隆 9B1-2G6, monoclonal
形式 buffered aqueous solution
分子量 antigen ~17 kDa
species reactivity human
浓度 ~1 mg/mL
technique(s) immunoblotting: 2-4 μg/mL using histones isolated from human HeLa cells
immunofluorescence: 2-4 μg/mL using human HeLa cells
同位素/亚型 IgG1
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
闪点(F) Not applicable
闪点(C) Not applicable

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