Anti-UBE2O antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

The gene UBE2O (ubiquitin conjugating enzyme E2 O) encodes a member of the E2 family of ubiquitin-conjugating enzymes. The encoded protein is large with a molar mass of 140kDa. It contains a ubiquitin-conjugating (UBC) domain and is expressed ubiquitously, with increased expression in brain, skeletal muscle, and heart tissues. It is found to be up-regulated during the reticulocyte stage of erythroid differentiation.

免疫原

ubiquitin-conjugating enzyme E2O recombinant protein epitope signature tag (PrEST)

应用

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

生化/生理作用

The E2 enzyme encoded by the gene UBE2O (ubiquitin conjugating enzyme E2 O) negatively regulates TRAF6-mediated IL-1R (interleukin-1R)/TLR4 (toll-like receptor4) signaling via the inhibition of polyubiquitination of TRAF6 (tumor necrosis factors receptor associated factor 6). It hinders the formation of MyD88-TRAF6 protein complex. It prevents TRAF6-dependent NF-κB (nuclear factor-κB) signaling. It is involved in the ubiquitination of the nuclear localization signal of BAP1 (BRCA1 associated protein 1), a tumor suppressor that regulates cell proliferation. UBE2O interacts and monoubiquitinates SMAD6, an inhibitory regulator of bone morphogenetic protein (BMP)/SMAD signaling, thereby regulating BMP7 (bone morphogenetic protein)-induced adipogenesis.

特点和优势

Prestige Antibodies^?are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^?to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

联系

Corresponding Antigen APREST86463.

外形

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

法律信息

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

人类蛋白质图谱编号

HPA023605 Human Protein Atlas characterization data

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous glycerol solution
species reactivity	human
包装	antibody small pack of 25 μL
technique(s)	immunoblotting: 0.04-0.4 μg/mL immunofluorescence: 0.25-2 μg/mL immunohistochemistry: 1:20-1:50
免疫原序列	GVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFF
UniProt登记号	Q9C0C9
运输	wet ice
储存温度	?20℃
Gene Information	human ... UBE2O(63893)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable