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位置: 首页> 品牌> Sigma-Aldrich> Anti-Tubulin β Antibody, clone 5H1

Anti-Tubulin β Antibody, clone 5H1

品牌
Sigma-Aldrich
货号
MAB380
规格纯度
ascites fluid, clone 5H1, Chemicon?
价格
5520.38 *本价格含增值税费
促销
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半岛bd体育手机客户端 名称:
Anti-Tubulin β Antibody, clone 5H1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

特异性

Beta tubulin

免疫原

Rat tubulin.

应用

Research Category
Cell Structure
Anti-Tubulin Antibody, β, clone 5H1 is an antibody against Tubulin for use in IH.
Research Sub Category
Cytoskeleton
Immunohistochemistry at 1:300-1:1,000

Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB380

This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).

外形

Ascites fluid containing sodium azide. Liquid

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 ascites fluid
antibody product type primary antibodies
克隆 5H1, monoclonal
species reactivity rat, bovine, human
manufacturer/tradename Chemicon?
technique(s) immunohistochemistry: suitable
同位素/亚型 IgM
NCBI登记号 NM_006086.2
UniProt登记号 Q13509
运输 dry ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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