Anti-Tryptase Antibody, Mast Cell, clone G3

半岛bd体育手机客户端 说明

一般描述

Tryptase is the most abundant secretory granule-derived serine proteinase contained in mast cells. Tryptase has recently been used as a marker for mast cell activation and is involved with allergic response. Tryptase may act as a mitogen for fibroblast lines. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis.

特异性

Recognizes Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.

应用

Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-Tryptase Antibody, Mast Cell, clone G3 is validated for use in FC, IH, WB for the detection of Tryptase.
Flow Cytometry Analysis: A previous lot of this antibody has been used on fixed cells in FACS using a conjugated secondary antibody. (Sperr, W., et al., 2001).

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology

质量

Evaluated by western blot on human fetal skin lysate.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected tryptase in 10 ?g of human fetal skin lysate.

目标描述

31 kDa

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
(see product datasheet for specific buffer formulation)
Format: Purified
Protein G Purified

储存及稳定性

Maintain at 2–8°C for the duration listed on the product datasheet. (See product datasheet for storage conditions)

分析说明

Control
Mast cells, basophils

Human fetal skin lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
克隆	G3, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	flow cytometry: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NM_003294.3
UniProt登记号	Q15661
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable