半岛bd体育手机客户端 说明
一般描述
Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Phosphorylation of histones may also produce similar repressive effects depending on the cellular context. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
特异性
Broad species reactivity expected due to similar sequence homology.
This antibody recognizes Histone H3 trimethylated at Lys36.
免疫原
KLH-conjugated linear peptide corresponding to human Histone H3 trimethylated at Lys36.
Epitope: Trimethylated Lys36
应用
Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-trimethyl-Histone H3 (Lys36) (1:2,000 dilution).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Histone H3 in A431 cells.
ChIP-Sequencing: Chromatin immunoprecipitation was performed using the Magna ChIP
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Anti-trimethyl-Histone H3 (Lys36) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated at lysine 36. Also known as Anti-H3K36me3, this purified Ab is specificity verified by dot blot (DB) and has been validated in WB, ICC, DB, ChIP, ChIP-seq.
质量
Evaluated by Western Blot in HeLa cell extract and recombinant Histone H3 protein.
Western Blot Analysis: A 1:500 dilution of this antibody detected Histone H3 on 10 ?g of HeLa cell extract and recombinant Histone H3 protein.
目标描述
~17 kDa observed. An uncharacterized band appears at ~52 kDa in some lysates.
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa cell extract and recombinant Histone H3 protein
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human, mouse |
| technique(s) | ChIP: suitable (ChIP-seq) dot blot: suitable immunocytochemistry: suitable western blot: suitable |
| NCBI登记号 | NP_003484 |
| UniProt登记号 | Q16695 |
| 运输 | wet ice |
| Gene Information | human ... HIST1H3F(8968) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
