Anti-TRA-1-81 Antibody, clone TRA-1-81

半岛bd体育手机客户端 说明

一般描述

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.

特异性

This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.

免疫原

Human embryonal carcinoma cell line 2102Ep

应用

Immunofluorescence: A previous lot of this antibody was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 ?g/mL is suggested.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Pluripotent & Early Differentiation
Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.
Research Category
Stem Cell Research

目标描述

420/250 kDa

外形

Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.
Format: Purified
Ammonium Sulfate Precipitation

储存及稳定性

Stable for 1 year at from date of receipt.

分析说明

Control
Human embryonic stem cells

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	saturated ammonium sulfate (SAS) precipitated
antibody product type	primary antibodies
克隆	TRA-1-81, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	flow cytometry: suitable immunocytochemistry: suitable immunofluorescence: suitable immunoprecipitation (IP): suitable western blot: suitable
input	sample type induced pluripotent stem cell(s) sample type: human embryonic stem cell(s)
同位素/亚型	IgM
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable