Anti-TIF1-alpha Antibody

半岛bd体育手机客户端 说明

一般描述

Transcription intermediary factor 1-alpha (TIF1-alpha) is also called E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, and Tripartite motif-containing protein 24. TIF1-alpha is a transcriptional coactivator that interacts with chromatin depending on histone H3 modifications, with the highest affinity for histone H3 that is both unmodified at ′Lys-4′ (H3K4me0) and acetylated at ′Lys-23′ (H3K23ac). TIF1-alpha has E3 protein-ubiquitin ligase activity, helps regulate cell proliferation and apoptosis and up-regulates transcription activation. Aberrant TIF1-alpha can cause thyroid papillary carcinoma (TPC) and is up-regulated in some breast cancers.

特异性

Other homologies: Rat (93% sequence homology).
This antibody recognizes the C-terminus of the Nuclear Receptor Binding Site (NRBS) of TIF1-alpha.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of the Nuclear Receptor Binding Site (NRBS) of human TIF1-alpha.
Epitope: C-terminus of the Nuclear Receptor Binding Site (NRBS)

应用

Immunocytochemistry Analysis: A 1:50 dilution from a represenative lot detected TIF1-alpha in HeLa cells.
Detect TIF1α using this rabbit polyclonal antibody, Anti-TIF1-alpha Antibody validated for use in western blotting & ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.1 ?g/mL of this antibody detected TIF1-alpha in 10 ?g of HeLa cell lysate.

目标描述

~140 kDa observed. Uniprot descrives 2 isoforms at ~117 kDa and ~113 kDa due to alternative splicing. This protein has been observed at ~140 kDa (Gunawardena, H., et al. (2008). Rheumatology (Oxford). 47(3):324-328.).

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	mouse, human
species reactivity (predicted by homology)	rhesus macaque (based on 100% sequence homology)
technique(s)	immunocytochemistry: suitable western blot: suitable
NCBI登记号	NP_056989
UniProt登记号	O15164
运输	wet ice
Gene Information	human ... TRIM24(8805)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable