半岛bd体育手机客户端 说明
一般描述
TGN46 is one of several proteins that utilize retrograde transport to travel between the trans-Golgi network (TGN) and the endocytic pathway. Retrograde transport involves the tethering, docking, fusion, and post translational modification of TGN proteins. Further studies are still required to elucidate the roles and mode of action for TGN46.
特异性
This antibody recognizes the cytoplasmic domain of TGN46.
免疫原
Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TGN46.
应用
Research Category
Cell Structure
Western Blot Analysis: 2 ?g/mL of this antibody detected TGN46 in 10 ?g of NIH/3T3 cell lysate. Multiple uncharacterized bands may be observed in some cell lysates.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected TGN46 in A431 and HeLa cells.
Research Sub Category
Vesicular Trafficking
Anti-TGN46 detects levels of TGN46 proteins & has been published & validated for use in wb, IP & ICC.
质量
Evaluated by Immunoprecipitation in HeLa cell lysate.
Immunoprecipitation Analysis: 0.2 ?g of this antibody immunoprecipitated TGN46 in 500 ?g of HeLa cell lysate (An uncharacterized band at ~29 kDa may be observed in some cell lysates.).
目标描述
~110 kDa observed. This protein is observed at ~110 kDa, and it is subject to glycosylation (Khanobdee, K. et al. (2004). Mol Vis. 13(10):933-42.; Prescott, A. R., et al. (1997). Eur J Cell Biol. 72(3):238-246.).
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | rat, mouse, human |
| species reactivity (predicted by homology) | canine (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| NCBI登记号 | NP_006455 |
| UniProt登记号 | O43493 |
| 运输 | wet ice |
| Gene Information | human ... TGOLN2(10618) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
