Anti-Tenascin Antibody, clone DB7

半岛bd体育手机客户端 说明

特异性

The antibody recognizes the Mr 250000 and 180000 tenascin polypeptides in immunoprecipitation and immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein

免疫原

Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography.

应用

Research Category
Cell Structure
Research Sub Category
ECM Proteins
Detect Tenascin using this Anti-Tenascin Antibody, clone DB7 validated for use in IF, WB, IH(P).
Immunofluorescence: 1-2% PFA fixed, permeabilized tissues or cell cultures. Mild pepsin digestion improves staining.

Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.

Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.

Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)

Final dilutions must be determined by end user.

联系

Replaces: MAB1902

外形

The purified antibody is in PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.
Format: Purified

储存及稳定性

Maintain refrigerated at 2-8°C

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	DB7, monoclonal
species reactivity	guinea pig, human, rabbit
should not react with	rat, mouse
manufacturer/tradename	Chemicon?
technique(s)	immunofluorescence: suitable immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG2a
UniProt登记号	P24821
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable