Anti-Tapasin Antibody, clone 7F6

半岛bd体育手机客户端 说明

一般描述

Tapasin (TPN or TPSN) is also known as NGS-17, TAP-associated protein and TAP-binding protein. Tapasin plays a role in MHC class I peptide loading and assembly, and is involved in associating MHC class I with transporter associated with antigen processing (TAP). Tapasin is found in neutrophils, predominantly within fully differentiated cells.

特异性

This antibody recognizes the Lumenal domain of Tapasin.

免疫原

Linear peptide corresponding to human Tapasin.

应用

Detect Tapasin using this rat monoclonal antibody, Anti-Tapasin Antibody, clone 7F6 validated for use in western blotting & Flow Cytometry.
Western Blotting Analysis: 0.5 ?g/mL from a representative lot detected Tapasin in 10 ?g of A431, HEK293, HepG2, HUVEC, Jurkat, and PC12 cell lysates.

Flow Cytometry Analysis: 4 ?g from a representative lot detected Tapasin in 1X10E6 PBMC cells.

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA transfected MJS cell lysate (Hislop, A. D., et al. (2007). J Exp Med. 204(8):1863-1873.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in HeLa cell lysate (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA4M transfected MJS cell lines (Horst, D., et al. (2009). J Immunol. 182(4):2313-2314.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in WB (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Tapasin in 10 ?g of HeLa cell lysate.

目标描述

~48 kDa observed

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	7F6, monoclonal
species reactivity	human
technique(s)	flow cytometry: suitable
同位素/亚型	IgG1κ
NCBI登记号	np_003181
UniProt登记号	O15533
运输	wet ice
Gene Information	human ... TAPBP(6892)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable