Anti-Staphylococcus aureusLTA antibody, Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Staphylococcus aureus is a Gram-positive bacterium, that causes disease in the human population including food poisoning and toxic shock syndrome.1S. aureusexpresses several secreted virulence factors, such as various enzymes, cytotoxins, exotoxins, and exfoliative toxins.1 Exotoxins include more than 20 serologically classified staphylococcal enterotoxins (SEs). The best characterized are the SEs A, B through V, and toxic shock syndrome toxin-1 (TSST-1). These enterotoxins are similar in activity, sequence, structure, and molecular mass (25-30 kDa).1-3

特异性

Monoclonal Anti-Staphylococcus aureuslipoteichoic acid (LTA) antibody specifically recognizes LTA ofStaphylococcus aureusand has no cross reactivity withBacillus subtilisLTA andEnterococcus hiraeLTA.

应用

The antibody may be used in various immunochemical techniques including Immunoblotting and ELISA.

生化/生理作用

SEA and SEB are known as superantigens due to their ability to bind class II MHC molecules on antigen presenting cells. This binding stimulates extensive T-cell activation followed by massive cytokine release leading to an acute toxic shock.1-4 SEB is also classified as a category B select agent and is important toxicant in biodefense research.5-9SE proteins have a significant resistance to heat and acid, and are also resistant to gastrointestinal proteases including pepsin, trypsin, rennin, and papain. Thus, inactivating the S. aureus bacteria may not be sufficient to eliminate the risk of its superantigens from causing food poisoning.1 SEB contamination can be caused by both its ingestion (resulting with food poisoning) or by inhalation (resulting with respiratory symptoms of cough and dyspnea), therefore SEB detection is a major challenge in the food industry.1,3,5-10

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.

免责声明

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

半岛bd体育手机客户端 性质

质量水平	200
抗体形式	purified from hybridoma cell culture
克隆	LT-43
形式	liquid
species reactivity	Staphylococcus aureus
浓度	~1 mg/mL
同位素/亚型	IgM
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1