Anti-SPRY1 Antibody, clone 7E6.2

半岛bd体育手机客户端 说明

一般描述

Protein sprouty homolog 1 (UniProt O43609; also known as Spry-1) is encoded by the SPRY1 gene (Gene ID 10252) in human. Sprouty was originally identified in Drosophila as a negative regulator of fibroblast growth factor (FGF) signaling during tracheal development. There exist four mammalian homologs (SPRY1-4) that likewise mediate feedback inhibition against growth factor-/receptor tyrosine kinase- (RTK-) dependent Ras/mitogen-activated protein (MAP) kinase signaling pathway. Loss of function studies in mice show that SPRY1 and SPRY2 play critical roles during early development of multiple organ rudiments, including external genitalia, eye, kidney, inner ear, tooth, and cortical patterning in the brain. In addition, evidences indicate that SPRY1 plays an antiproliferative and tumor-suppressing role in cancers. SPRY1 downregulation is reported in various cancer types, including prostate, breast, and ovarian cancers, and SPRY1 expresion has been proposed as a marker of good prognosis in patients with renal cell carcinoma.

特异性

Clone 7E6.2 detects the ~35 kDa human SPRY1 by targeting an N-terminal region epitope.

免疫原

GST-tagged recombinant human SPRY1 N-terminal fragment.

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SPRY1 in 10 ?g of SK-MEL-2 human skin melanoma cell lysate.

Research Category
Stem Cell Research
This mouse monoclonal Anti-SPRY1 Antibody, clone 7E6.2, Cat. No. MABD390 is validated for use in Western Blotting for the detection of SPRY1.

质量

Evaluated by Western Blotting in HepG2 cell lysate.


Western Blotting Analysis: A 1:1,000 dilution of this hybridoma culture supernatant detected SPRY1 in 10 ?g of HepG2 human hepatocellular carcinoma cell lysate.

目标描述

~35 kDa observed. 35.12 kDa calculated.

外形

Unpurified
Mouse IgG1 hybridoma culture supernatant without azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	culture supernatant
antibody product type	primary antibodies
克隆	7E6.2, monoclonal
species reactivity	human
technique(s)	western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_001244967
UniProt登记号	O43609
运输	ambient

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1