半岛bd体育手机客户端 说明
一般描述
Non-erythroid Spectrin, also known as Fodrin, plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. As one of the primary targets cleaved by activated caspases during apoptosis, the full-length ~240 kDa protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa.
特异性
Specific for the 240, 150, and 120 kDa forms of alpha-spectrin/alpha-fodrin molecule of all mammalian nonerythroid cells and chicken alpha-spectrin.
Wide-mammalian reactivity is expected.
免疫原
Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation.
应用
Research Category
Cell Structure
Immunohistochemistry (IFA):
A 1:200-1:400 dilution of a previous lot was used in immunohistochemistry.
Does not work on paraffin sections.
Optimal working dilutions must be determined by end user.
Chromatin Immunopreciptiation:
A previous lot of this antibody was used to immunoprecipitate Spectrin Alpha from chromatin-associated protein extracts derived from normal human, HeLa, FA-A, and transduced FA-A cells (McMahon, L.W., et al. (1999). J. Biological Chem. 274:32904-32908).
Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6 is an antibody against Spectrin alpha chain (nonerythroid) for use in IH, IP, WB, ChIP.
Research Sub Category
Cytoskeleton
质量
Evaluated by Western Blot on staurosporine-treated HeLa lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected N-, C- terminal Spectrin alpha/Fodrin alpha on 10 μg of staurosporine treated HeLa lysates.
目标描述
~240 kDa full length product, ~150 kDa N-terminal cleaved product and ~120 kDa C-terminal product may be seen in varing amounts in lysates, depending on condition. The smaller sizes represent break down products seen during apoptosis.
外形
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.09% sodium azide.
储存及稳定性
Stable for 12 months at 2-8°C from date of receipt.
分析说明
Control
HeLa cells treated with staurosporine
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | AA6, monoclonal |
species reactivity | pig, rat, human, rabbit, guinea pig, chicken, mouse |
species reactivity (predicted by homology) | mammals |
manufacturer/tradename | Chemicon? |
technique(s) | ChIP: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1 |
适用性 | not suitable for immunohistochemistry (Paraffin) |
NCBI登记号 | NM_003127.2 |
UniProt登记号 | Q13813 |
运输 | wet ice |
Gene Information | human ... SPTAN1(6709) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich