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位置: 首页> 品牌> Sigma-Aldrich> (ALPHA)II-SPECTRIN, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha chain, non-erythrocytic spectrin alpha, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Fodrin α

Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6

品牌
Sigma-Aldrich
货号
MAB1622
规格纯度
clone AA6, Chemicon?, from mouse
价格
7701.24 *本价格含增值税费
促销
服务
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  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6
(ALPHA)II-SPECTRIN, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha chain, non-erythrocytic spectrin alpha, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Fodrin α
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Non-erythroid Spectrin, also known as Fodrin, plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. As one of the primary targets cleaved by activated caspases during apoptosis, the full-length ~240 kDa protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa.

特异性

Specific for the 240, 150, and 120 kDa forms of alpha-spectrin/alpha-fodrin molecule of all mammalian nonerythroid cells and chicken alpha-spectrin.
Wide-mammalian reactivity is expected.

免疫原

Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation.

应用

Research Category
Cell Structure
Immunohistochemistry (IFA):
A 1:200-1:400 dilution of a previous lot was used in immunohistochemistry.
Does not work on paraffin sections.

Optimal working dilutions must be determined by end user.

Chromatin Immunopreciptiation:
A previous lot of this antibody was used to immunoprecipitate Spectrin Alpha from chromatin-associated protein extracts derived from normal human, HeLa, FA-A, and transduced FA-A cells (McMahon, L.W., et al. (1999). J. Biological Chem. 274:32904-32908).
Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6 is an antibody against Spectrin alpha chain (nonerythroid) for use in IH, IP, WB, ChIP.
Research Sub Category
Cytoskeleton

质量

Evaluated by Western Blot on staurosporine-treated HeLa lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected N-, C- terminal Spectrin alpha/Fodrin alpha on 10 μg of staurosporine treated HeLa lysates.

目标描述

~240 kDa full length product, ~150 kDa N-terminal cleaved product and ~120 kDa C-terminal product may be seen in varing amounts in lysates, depending on condition. The smaller sizes represent break down products seen during apoptosis.

外形

Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.09% sodium azide.

储存及稳定性

Stable for 12 months at 2-8°C from date of receipt.

分析说明

Control
HeLa cells treated with staurosporine

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 AA6, monoclonal
species reactivity pig, rat, human, rabbit, guinea pig, chicken, mouse
species reactivity (predicted by homology) mammals
manufacturer/tradename Chemicon?
technique(s) ChIP: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG1
适用性 not suitable for immunohistochemistry (Paraffin)
NCBI登记号 NM_003127.2
UniProt登记号 Q13813
运输 wet ice
Gene Information human ... SPTAN1(6709)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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