Anti-SIP1 Antibody, clone 6E5

半岛bd体育手机客户端 说明

一般描述

Smad-interacting protein 1 (SIP1, SMADIP1), also known as Zinc finger E-box-binding homeobox 2 (ZEB2), and Zinc finger homeobox protein 1b (ZFHX1B, ZFX1B), is a transcriptional inhibitor that binds to DNA sequence 5′-CACCT-3′ in different promoters and interacts with activated SMADs. ZEB2 has been shown to repress the transcription of the E-cadherin gene and induce epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing in vitro. It is suggested that ZEB2 contributes to tumor progression since it’s been demonstrated to protect cells from DNA damage-induced apoptosis. Recent studies have shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, implying that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Mowat-Wilson syndrome (MWIS), also known as Hirschsprung disease-mental syndrome.

免疫原

His-tagged recombinant protein corresponding to the N-terminus of Human SIP1.
Epitope: N-terminus

应用

This antibody has been shown to work in Immunocytochemistry and Immunohistochemistry applications (Oztas et al., 2010).
Anti-SIP1 Antibody, clone 6E5 is an antibody against SIP1 for use in western blotting, ICC & IHC.
Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research

质量

Evaluated by Western Blot in a SK-Hep1 cell lysate. 2ug/ml (1:500 dilution) of this antibody detected SIP-1 in a SK-Hep1 cell lysate.

目标描述

~220 kDa observed

外形

Purified mouse monoclonal IgG2aκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	6E5, monoclonal
species reactivity	human
浓度	1 mg/mL
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_001165124
UniProt登记号	O60315
运输	wet ice
Gene Information	human ... ZEB2(9839)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable