Anti-Sigma IR Antibody, clone ICR-SIG1R-A

半岛bd体育手机客户端 说明

一般描述

Sigma1R, or Sigma non-opioid intracellular receptor 1, is part of the ERG2 family that forms a ternary complex with ANK2 and ITPR3. Sigma1R is involved in lipid transport from the endoplasmic reticulum, BDNF and EGF signaling, potassium ion channel regulation and other cell functions including apoptosis, survival and proliferation. It was initially discovered through its binding to various psychoactive drugs, and has been studied in memory, learning and mood alteration processes. Sigma1R is often found in the nuclear membrane, synapses and endoplasmic reticulum of cells within liver, brain, kidney and thymus tissues.

免疫原

Linear peptide corresponding to human Sigma IR.

应用

Detect the Sigma IR protein using this Anti-Sigma IR, clone ICR-SIG1R-A validated for use in wb, IP, ICC, Flow & IHC.
Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in certain cancer cell lysates.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.
Flow Cytometry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in tumor cells.
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blotting in colo-587 cell lysate.

Western Blotting Analysis: 1.0 ?g/mL of this antibody detected Sigma IR in 10 ?g of colo-587 cell lysate.

目标描述

~25 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing at ~25 kDa, ~23 kDa, ~21.5 kDa, ~12 kDa and ~21 kDa

外形

Format: Purified
Purified rat monoclonal IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Colo-587 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	ICR-SIG1R-A, monoclonal
species reactivity	human
species reactivity (predicted by homology)	mouse (immunogen homology)
technique(s)	flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG2c
NCBI登记号	NP_005857
UniProt登记号	Q99720
运输	wet ice
Gene Information	human ... SIGMAR1(10280)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable