Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal

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一般描述

Monoclonal Anti-Shiga Toxin 1, B Subunit (mouse IgG1 isotype) is derived from the 13C4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a purified Shiga-like toxin fromE. coliH30. Shiga toxins consist of two polypeptides, an A chain and a B chain non-covalently associate with an apparent stoichiometry of one A and five B chains to form the holotoxin.
The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced byShigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains ofEscherichia colinamed Shiga-toxin-producingE. coli(STEC) such asE. coliO157:H7. The latter may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications.

特异性

Monoclonal Anti-Shiga Toxin 1, B Subunit antibody specifically recognizes the B subunit of Shiga holotoxin.

免疫原

purified Shiga-like toxin from E.coli H30

应用

Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal has been used in:

• immunoblotting
• flow cytometry
• immunoprecipitation

生化/生理作用

Shiga-toxin (Stx) produced byE. coliO157:H7 may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. All the Stx isoforms have similar structure and mechanism of action. The catalytic A subunit has RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide [Gb3, Gala(1-4)-Galb (1-4)-Glcb1-ceramide]. Gb3 is overexpressed in membranes of numerous tumor cells, Therefore, STxB binding to Gb3 receptors may be useful for cell-specific vectorization, labeling, and imaging purposes.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

其他说明

This product is for R&D use only, not for drug, household, or other uses.

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