Anti-SF3b155 (SAP 155) Antibody

半岛bd体育手机客户端 说明

一般描述

Splicing factor 3B subunit 1, also known as Pre-mRNA-splicing factor SF3b 155 kDa subunit (SF3b155), Spliceosome-associated protein 155 (SAP 155), and encoded by the gene name SF3B1 or SAP155, is a member of the SF3B1 family . SF3B155 (SAP 155) functions a s a subunit of the splicing factor SF3B required for ′A′ complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B155 (SAP 155) has been implied to be involved in the assembly of the ′E′ complex, and also may anchor U2 snRNP to the pre-mRNA. Additionally, SF3B155 (SAP 155) belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. SF3B155 (SAP 155) has been identified in the spliceosome C complex, as a component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Phosphorylation of SF3B155 (SAP 155) occurs concomitantly with the splicing catalytic steps. The phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis. SF3B155 (SAP 155) is located in the nucleus speckle and during mitosis, it is transiently dispersed from the nuclear speckles to the cytoplasm.

免疫原

Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to human SF3b155 (SAP 155) near the N-terminus.

应用

Research Sub Category
Nuclear Receptors
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SF3b155 (SAP 155) in HeLa, A431, and NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Anti-SF3b155 (SAP 155) is an antibody against SF3b155 (SAP 155) for use in WB, ICC.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SF3b155 (SAP 155) in 10 ?g of HeLa cell lysate.

目标描述

~160 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	rat, mouse, human
technique(s)	immunocytochemistry: suitable western blot: suitable
NCBI登记号	NP_036565
UniProt登记号	O75533
运输	wet ice
Gene Information	human ... SF3B1(23451)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable