Anti-Septin-9 Antibody, clone 1A6C2

半岛bd体育手机客户端 说明

一般描述

Septin-9 (also known as eighth septin, Eseptin, and MLL septin-like fusion) is encoded by the Sept9 gene in rat and mouse (SEPT9 in human). Septins are GTP-binding proteins capable of forming higher-order oligomers and filamentous polymers that contact with the cell membrane and cytoskeleton. Septins are involved in various cellular processes, including cytokinesis, karyokinesis, exocytosis, and cell shape maintenance. The expression of SEPT2, SEPT6, SEPT7 and SEPT9 in heart tissue is reported to correlate well with cardiomyocyte cytokinesis activity during heart development, is highly expressed in embryonic heart, downregulated around birth, and nondetectable in the adult heart. (PMID 16739252 & 24837489). Clone 1A6C2 was generated using an immunogen sequence present only in rat spetin-9 isoform 1 (SLP-a; Q9QZR6), but missing in rat isoform 2-4 (SLP-b, E-septin long & short forms). The immunogen sequence is 100% conserved in murine species (A2A6U3) and differs by one amino acid when compared to the corresponding human sequence in isoform 2, and missing in human isoforms 1,3,4 (Q9UHD8).

免疫原

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of rat Septin-9.

应用

Research Category
Cell Structure
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Septin-9 in HeLa cells (Dr. Taro Tachibana, Cell Engineering Corporation).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Septin-9 in HeLa cell lysate (Dr. Taro Tachibana, Cell Engineering Corporation).
Research Sub Category
Adhesion (CAMs)
Anti-Septin-9 Antibody, clone 1A6C2 is an antibody against Septin-9 for use in Western Blotting and Immunocytochemistry.

质量

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected Septin-9 in 10 ?g of NIH/3T3 cell lysate.

目标描述

~65 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	1A6C2, monoclonal
species reactivity	mouse, human
technique(s)	immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_001106969
UniProt登记号	Q9QZR6
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable