Anti-Sec8 Antibody/Exoc4, clone 2E12

半岛bd体育手机客户端 说明

一般描述

Sec8/Exoc4 is a component of the exocyst complex which is a multi-protein complex used for docking of exocytic vesicles with fusion sites on the plasma membrane. The exocyst complex is essential for exocytosis which is the process whereby proteins, hormones, transmitters etc. are transported and released to the extracellular environment. The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Sec8/Exoc4 appears to be important for tethering of secretory vesicles to specific regions on the plasma membrane. It seems particularly important in normal development for growth and morphological change by allowing the secretion of matrix metalloproteinases such as MMP2 or MMP9 in discrete locations. Knockdowns exhibit reduced cellular proliferation as well as invasive activities as well. As such Sec8/Exoc4 is important in tumor growth and invasion as well, thus it is also a current target for cancer therapy.

免疫原

Recombinant protein corresponding to rat Sec8/Exoc4.

应用

This Anti-Sec8 Antibody/Exoc4, clone 2E12 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sec8/Exoc4.
Research Sub Category
Apoptosis - Additional
Immunocytochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sec8/Exoc4 in HeLa, A431, and NIH/3T3 cells. DyLight^?is a registered trademark of Thermo Fisher Scientific.
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells treated with EXO70 SiRNA (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in B16F1 cells treated with siRNA targeting Sec8 (Zhao, Y., et al. (2013). Dev. Cell. 26:266-278).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells transfected with GFP-Exo70 (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in Rat emreyonic hippocampal neurons (Menchon, S.A., et al. (2011). PLOS ONE. 6(9):e24190 1-11).
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Sec8/Exoc4 in 10 ?g of HeLa cell lysate.

目标描述

~110 kDa observed

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

法律信息

DyLight is a registered trademark of Pierce Biotechnology, Inc.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	2E12, monoclonal
species reactivity	mouse, rat, human
technique(s)	immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_446327
UniProt登记号	Q62824
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable