半岛bd体育手机客户端 说明
一般描述
Members of the F-box protein family, such as SCRAPPER, are characterized by an approximately 40-amino acid F-box motif. SCRAPPER is selectively expressed in the brain, broadly expressed within the mouse CNS, and is abundant at the presynaptic membrane. It has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. SCRAPPER is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.
免疫原
KLH-conjugated linear peptide corresponding to human SCRAPPER.
应用
This SCRAPPER antibody is validated for use in WB & IHC for the detection of the SCRAPPER protein.
Western Blot Analysis: 1 ?g/mL from a representative lot detected SCRAPPER in 10 ?g/mL of RAW264.7 cell lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected SCRAPPER in human midbrain tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
质量
Evaluated by Western Blot in human spleen tissue lysate.
Western Blot Analysis: 1 ?g/mL of this antibody detected SCRAPPER in 10 ?g/mL of human spleen tissue lysate.
目标描述
~48 kDa observed. Uncharacterized bands may appear at ~52 kDa, ~58 kDa and ~70 kDa in some lysates.
外形
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
Antigen Affinity Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Human spleen tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable western blot: suitable |
| UniProt登记号 | Q96IG2 |
| 运输 | wet ice |
| Gene Information | human ... FBXL20(84961) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
