Anti-SCAMP2 antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

SCAMP2 (secretory carrier membrane protein 2) belongs to the SCAMP family of highly conserved integral membrane proteins, which are involved in cell surface recycling pathways). SCAMP2 is one of the two isoforms of SCAMP, the other being SCAMP1, and has a molecular weight of 39kDa. It has a wide range of tissue expression. It is a transmembrane protein, which contains a cytoplasmic N-terminal, cytoplasmic C-terminal tail and four transmembrane regions. It contains NHE5 (Na⁺/H⁺exchanger)-binding sites in both its N- and C-termini. It also contains the characteristic E-peptide of SCAMP proteins, within the second and third transmembrane helices. This protein resides in endosomes, other recycling organelles, as well as plasma membrane.

免疫原

Secretory carrier-associated membrane protein 2 recombinant protein epitope signature tag (PrEST)

应用

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

生化/生理作用

SCAMP2 (secretory carrier membrane protein 2) is involved in Ca²⁺-dependent granule exocytosis, and is a part of the later stages of membrane fusion. It controls the formation of membrane fusion pores, by sequestering phosphatidylinositol 4,5-bisphosphate (PIP2) within membranes. It interacts with PIP2 via a hydrophobic sequence CWYRPIYKAFR, present in its structure. It interacts with serotonin and dopamine transporters, and controls their subcellular distribution and their surface expression. It interacts with NHE5, which is a Na⁺/H⁺exchanger highly expressed in brain, through its cytoplasmic N- and C-termini. This way, it regulates the movement of NHE5 through recycling endosomes, and its cell surface targeting, in the presence of Arf6 (ADP-ribosylation factor 6). The E-peptide of SCAMP2 protein is a potent inhibitor of exocytosis during the later stages.

特点和优势

Prestige Antibodies^?are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^?to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

联系

Corresponding Antigen APREST73121.

外形

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

法律信息

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD04040583
人类蛋白质图谱编号	HPA014699 Human Protein Atlas characterization data

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
半岛bd体育手机客户端 线	Prestige Antibodies?Powered by Atlas Antibodies
形式	buffered aqueous glycerol solution
species reactivity	mouse, human, rat
包装	antibody small pack of 25 μL
technique(s)	immunoblotting: 0.04-0.4 μg/mL immunofluorescence: 0.25-2 μg/mL immunohistochemistry: 1:200-1:500
免疫原序列	DPVDVNPFQDPSVTQLTNAPQGGLAEFNPFSETNAATTVPVTQLPGSSQPAVLQPSVEPTQPTPQAVVSAAQAGLLRQQEELDRKAAELERKERELQNTVANLHV
UniProt登记号	O15127
运输	wet ice
储存温度	?20℃
Gene Information	human ... SCAMP2(10066)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)