半岛bd体育手机客户端 说明
一般描述
Salmonella is a genus of Gram-negative, facultative anaerobe, motile bacillus composed of 2579 different serotypes. Salmonella is considered to be one of the most studied microorganisms as a worldwide foodborne pathogen and a major cause of gastrointestinal human infections.1S. entericais a subspecies ofS. enterica, that includes 1531 serotypes, among themS. typhimuriumandS. enteritidis, responsible for common human illness and thus focus of extensive research.1
特异性
almonella enterica typhimurium antibody recognizes S. enterica typhimurium whole lysate of dead bacteria and S. enterica typhimurium Lipopolysaccharides (LPS), the antibody has no cross reactivity with whole bacterial extracts ofE. coliK-12 andE. faecalis.
应用
The antibody may be used in various immunochemical techniques including Immunoblotting and ELISA. Detection of the S. typhimurium bands by Immunoblotting is specifically inhibited by the immunogen.
生化/生理作用
S. typhimuriumcan survive the acidic environment ofthe stomach through the activation of acid tolerance response (ATR), which maintains the intracellular pH
higher than the extracellular pH.6 Then, S. typhimurium penetrates the mucus barrier of the intestinal wall and adheres to the epithelium to initiate the infection, that may results in diarrhea, loss of electrolytes and local inflammation of the intestine.7-9
AfterS. typhimuriumis being engulfed by enterocytes, it forms intracellular phagosomal compartments, termed Salmonella-Containing Vacuoles (SCV) where the bacterium is able to survive and multiply.2,9S. typhimuriumhas various virulence factors, most of them are enoced by genes located in the Salmonella Pathogenicity Islands (SPI).10S. typhimuriumuses flagella for motility after ingestion11 and then type I fimbrae12 and adhesins to adhere to the epithelium. It expresses Type III Secretion System (T3SS) that enables the bacteria to inject into the host cells a variety of effector proteins such as, SipABCD, SptP, SpiC and SopB that alter cellular functions to promote bacterial invasion, survival and propagation.2,13,14 Moreover,S. typhimuriumis able to form resistant biofilms15 that contribute to the establishment of chronic, ""carrier state"" infections. In addition, human specific S. typhiis used as a model for the animal study of typhoid fever.16
外形
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
储存及稳定性
For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses,in vitrodiagnostic uses,ex vivoorin vivotherapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 形式 | liquid |
| species reactivity | (Salmonella enterica typhimurium) |
| 包装 | pkg of 100 μL pkg of 25 μL |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: 1:2000-1:4000 usingSalmonella entericatyphimurium LPS indirect ELISA: 1:2000-1:4000 using using whole Salmonella |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
