Anti-Ryanodine Receptor Antibody, clone RYR.1

半岛bd体育手机客户端 说明

特异性

Reacts with the C-terminus cytoplasmic domain of ryanodine receptor. Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. MAB3086 reacts with ryanodine receptor (MW approx. 500kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues).

免疫原

Short synthetic polypeptide corresponding to the c-terminal domain of the ryanodine receptor.

应用

Research Category
Neuroscience
Anti-Ryanodine Receptor Antibody, clone RYR.1 detects level of Ryanodine Receptor & has been published & validated for use in ELISA, FC, IC, IH, IP, RIA & WB.
Immunoblot: 1:100 using enhanced chemiluminescence detection. It is suggested that you perform a dot blot to verify presence of the RyR protein if you are having difficulties performing an immunoblot assay.

Immunocytochemistry: 1:100 by indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the RyR. Additionally, fluorescent, not enzymatic, detection is required. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended.

Immunoprecipitation: 1:100

Flow cytometry: 1:500

ELISA: 1:1000

Immunohistochemistry: 1:100

RIA: 1:5000

Optimal working dilutions must be determined by the end user.
Research Sub Category
Ion Channels & Transporters

Signaling Neuroscience

外形

Purified immunoglobulin from culture supernatant. In 10 mM sodium phosphate, 150 mM NaCl, 0.01% NaN3, pH. 7.5
Format: Purified

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	RYR.1, monoclonal
species reactivity	canine, mouse, pig, rat, bovine, human
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable radioimmunoassay: suitable western blot: suitable
同位素/亚型	IgG2b
NCBI登记号	NM_000540.2 NM_001042723.1
UniProt登记号	P21817
运输	dry ice
Gene Information	human ... RYR1(6261)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable