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Anti-Rubicon antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200838
规格纯度
clone RB-145, purified from hybridoma cell culture
价格
2036.2 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-Rubicon antibody, Mouse monoclonal
Beclin-1 associated RUN domain containing protein
KIAA0226
Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
RUBCN
SCAR15
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Run domain Beclin-1-interacting and cysteine-rich domain-containing protein (Rubicon) also known as Beclin-1 associated RUN domain containing protein (Baron) or KIAA0226, is a negative regulator of endosomes maturation and endocytic trafficking.1-2 Rubicon knockdown studies showed enhanced autophagosome maturation and endocytosis.1 It also have a critical role in the LC3-associated phagocytosis (LAP) a noncanonical autophagy process which can be induced through the activation of extracellular receptor by different stimulators including pathogens, immune complexes and dying cells.4-5

特异性

Monoclonal Anti-Rubicon antibody specifically recognizes Rubicon from human origin.

免疫原

synthetic peptide corresponding to the N-terminal region of human Rubicon, conjugated to KLH

应用

The antibody may be used in various immunochemical techniques including Immunoblotting and Immunofluorescence. Detection of the Rubicon band by Immunoblotting is specifically inhibited by the immunogen.

生化/生理作用

The mammalian class III phosphatidylinositol 3-kinase (PtdIns3K) complex consists of three core proteins, catalytic VPS34, the adaptor VPS15 (p150) and the recruiter Beclin-1 (ATG6); these endocytosis regulators were reported to stably bind Beclin-1 ATG14, UVRAG and Rubicon.1-3 The complex Rubicon-UVRAG–Beclin-1/PtdIns3K can be found both in early and in late endosomes or lysosomes. Rubicon was described as the mediator for the recruitment of the UVRAG-Beclin-1-VPS34 complex and of ATG7 and LC3-II LAPosome.4-5 Furthermore, Rubicon has been identified as a key modulator of the inflammatory response and viral replication, during several viral infections such as hepatitis B virus (HBV). Rubicon expression is induced in negative regulation of the innate immune response resulting in enhances viral replication and possibly supporting viral immune evasion mechanism.4

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,ex vivoorin vivotherapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 mouse
抗体形式 purified from hybridoma cell culture
antibody product type primary antibodies
克隆 RB-145, monoclonal
形式 liquid
species reactivity human
包装 antibody small pack of 25 μL
浓度 ~1 mg/mL
technique(s) immunoblotting: 2-4 μg/mL using human A431 whole cell extract.
immunofluorescence: 2-4 μg/mL using human HeLa cells.
同位素/亚型 IgG1
UniProt登记号 Q92622
运输 dry ice
储存温度 ?20℃
Gene Information human ... RUBCN(9711)

安全信息

储存分类代码 12 - Non Combustible Liquids
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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