Anti-RSPO1 Antibody

半岛bd体育手机客户端 说明

一般描述

R-spondin-1 (RSPO1), or Roof plate-specific spondin-1 protein, is potent activator of the Wnt/beta-catenin signaling pathway leading to TCF/LEF (T cell-specific transcription factor/lymphoid enhancer factor) gene activation. RSPO1 operates on both the canonical Wnt/beta-catenin and non-canonical Wnt signaling pathways by acting as an inhibitor of ZNRF3 (zinc and ring finger 3) protein, a cell surface transmembrane E3 ubiquitin ligase, which is an important regulator of Wnt signaling. RSPO1 interacts with the extracellular domain of ZNRF3 and induces ZNRF3 membrane clearance. RSPO1 is also part of a family of secreted proteins that play critical roles in vertebrate development, including female sex development, and RSPO1 is required for early development of the gonads regardless of sex. Molecularly, RSPO1’s activities synergize with those of WNT4 and act to stabilize beta catenin which then activates its downstream targets. RSPO1 has been shown to be a potent mitogen for specific cell types such as gastrointestinal epithelium, and it is being examined as a key target for colon cancer therapy and for the treatment of mucositis (inflammation of the oral cavity, which is common among patients receiving chemotherapy or radiation for head and neck cancers). Recently RSPO1 was also shown to be a novel beta-cell line growth factor and a factor that promotes insulin secretion in a glucose independent fashion, thus making it a target for enhancing beta-cell function in patients with type 2 diabetes. Defects in RSPO1 can lead to female to male sex reversal and have been associated with the genetic disease PKKSCC (palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal). EMD Millipore’s Anti-RSPO1 rabbit polyclonal antibody has been tested in western blotting, immunohistochemistry, and immunofluorescence in human and rat samples.

免疫原

KLH-conjugated linear peptide corresponding to human RSPO1.

应用

Detect R-spondin-1 using this rabbit polyclonal antibody, Anti-RSPO1 Antibody validated for use in western blotting, IHC & Immunofluorescence.
Research Category
Signaling
Immunohistochemistry Analysis: 2.5 ?g/mL from a representative lot detected RSPO1 in human spleen tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected RSPO1 in human spleen cells.
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blotting in rat spleen tissue lysate.

Western Blotting Analysis: 2 ?g/mL of this antibody detected RSPO1 in 15 ?g of rat spleen tissue lysate.

目标描述

~37 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 3 isoforms at ~22 kDa, ~26 kDa, and ~29 kDa

外形

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Antigen Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Rat spleen tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	rat, human
technique(s)	immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable
UniProt登记号	Q2MKA7
运输	wet ice
Gene Information	human ... RSPO1(284654)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable