半岛bd体育手机客户端 说明
一般描述
E3 ubiquitin-protein ligase RNF43 (UniProt Q68DV7; also known as RING finger protein 43) is encoded by the RNF43 gene (Gene ID 54894) in human. RNF43 and the homologous ZNRF3 are cell-surface transmembrane E3 ubiquitin ligases upregulated upon Wnt/ -catenin signaling and act as potent Wnt signaling pathway negative feedback regulators by mediating ubiquitination of Frizzled (FZD) and LRP6 for lysosomal degradation. RNF43/ZNRF3 activity is counteracted by stem cell growth factor R-spondin that induces membrane ZNRF3/RNF43 clearance by binding ZNRF3/RNF43 and LGR4/LGR5. Physical interaction with Dishevelled (DVL) is essential for ZNRF3/RNF43 Wnt inhibitory activity, and DVL knockdown significantly increases cell surface levels of FZD and LRP6. In addition, RNF43 suppresses canonical and noncanonical Wnt signaling via distinct mechanisms. While interaction between the extracellular protease-associated (PA) domain and the cysteine-rich domain (CRD) of FZD and RNF43 intracellular RING finger domain is necessary for canonical Wnt/ -catenin signaling inhibition, interaction between FZD PDZ domain and RNF43 C-terminal cytoplasmic region is essential for suppressing noncanonical Wnt signaling. R-spondin overexpression through gene translocation and loss-of-function RNF43/ZNRF3 gene mutations are found in human cancers as means to escape from Wnt signaling negative feedback regulation. Human RNF43 is produced with a signal peptide (a.a. 1-23) sequence, which is posttranslationally removed to yiled the mature enzyme with an N-terminal extracellular domain (a.a. 24-197), a transmembrane domain (a.a. 198-218), and a large cytoplasmic region (a.a. 219-783) that contains an atypical RING-type zinc finger (a.a. 272-313) and a pro-rich (a.a. 569-760) region.
特异性
Clone 11C3.1 epitope lies within the cytoplasmic pro-rich region present in all four spliced isoforms of human RNF43 reported by UniProt (Q68DV7).
免疫原
GST-tagged recombinant human RNF43 fragment from the cytoplasmic pro-rich region.
应用
Detect RNF43 using this mouse monoclonal Anti-RNF43, clone 11C3.1 Antibody, Cat. No. MABC606, validated for use in Western Blotting.
Research Category
Apoptosis & Cancer
质量
Evaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: 1 ?g/mL of this antibody detected RNF43 in 10 ?g of HCT116 human colon carcinoma cell lysate.
目标描述
~95 kDa observed. Target band size appears larger than the calculated molecular weights of 83.20/85.72 kDa (isoform 1 mature/pro-form), 78.71/81.23 kDa (isoform 2 mature/pro-form), 72.11 kDa (isoform 3) 92.52/95.04 kDa (isoform 4 mature/pro-form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
外形
Protein G purified.
Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 11C3.1, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| 同位素/亚型 | IgG2bκ |
| NCBI登记号 | NP_001292473 |
| UniProt登记号 | Q68DV7 |
| 运输 | ambient |
| Gene Information | human ... RNF43(54894) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
