半岛bd体育手机客户端 说明
一般描述
RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.
免疫原
Replication Protein A purified from U293 cells
应用
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-Replication Protein A Antibody, clone RPA34-20 (mouse monoclonal antibody) validated in WB, ICC, IHC to detect Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Replication Protein A in HeLa and A431 cells.
Immunohistochemistry Analysis: A 1:5 dilution from a representative lot detected Replication Protein A in human colorectal adenocarcinoma tissues.
质量
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 ?g/mL of this antibody detected Replication Protein A in 10 ?g of HeLa cell lysate.
目标描述
~30 kDa observed. Uniprot describes three isoforms at ~29 kDa, ~30 kDa, and ~39 kDa
联系
Replaces: NA19L
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa cell lysate.B10
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | RPA34-20, monoclonal |
species reactivity | human |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_002937 |
UniProt登记号 | P15927 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich