Anti-Rad51 (Ab-1) Rabbit pAb

半岛bd体育手机客户端 说明

一般描述

Affinity purified rabbit polyclonal antibody. Recognizes the ~43 kDa Rad51 protein.
Recognizes the ~43 kDa Rad51 protein in ovarian tissue.
This Anti-Rad51 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Rad51 (Ab-1).
The bacterial RecA and the yeast RAD51 proteins are involved in homologous recombination and DNA repair. The human RAD51 protein is the homologue of these two proteins. While the modular structure of these proteins is conserved and the homology is very strong with the bacterial protein there are functional differences seen with RAD51 including an ATP hydrolysis rate 1/10 that seen with RecA. Although RAD51 is a nuclear protein and is largely absent from the cytoplasm and nucleoli, the protein lacks a nuclear localization signal and requires other proteins in a complex to be translocated into the nucleus. Treatment of cells with DNA synthesis inducing agents will cause an increase in the levels of RAD51 protein which then undergo a shift in distribution becoming focal in nature. This focal distribution may be blocked by inhibitors of transcription. RAD51 can be shown to interact directly with the chromosomes and during the process of meiosis, its distribution of RAD51 undergoes dynamic change. RAD51 has been shown to interact with a wide variety of different proteins that include the wild-type form of p53, BRCA2, and RAD52, which specifically modulates its activity.

免疫原

recombinant, human Rad51
Human

包装

100 μL in Plastic ampoule

应用

Immunoblotting (1:2500)
Immunoprecipitation (40 l/500g total protein from lysate)
Immunocytochemistry (1:500)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
A431 cells, HeLa cells

其他说明

Due to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.
Brendel, V., et al. 1997.J. Mol. Evol.44,528.
Boulikas, T., et al. 1997.Anticancer Res.17,843.
Gupta, R.C., et al. 1997.Proc. Natl. Acad. Sci. USA94,463.
Mizuta, R., et al. 1997.Proc. Natl. Acad. Sci. USA94,6927.
Sharan, S.K., et al. 1997.Nature386,304.
Li, M.J., et al. 1996.Proc. Natl. Acad. Sci. USA93,10222.
Shen, Z., et al. 1996.J. Biol. Chem.271,148.
Sturzbecher, H.W., et al. 1996.EMBO J.15,1992.
Tashiro, S., et al. 1996.Oncogene12,2165.
Ashley, T., et al. 1995.Chromosoma104,19.
Haaf, T., et al. 1995.Proc. Natl. Acad. Sci. USA92,2298.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	rat, human, mouse
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable