您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-Pyrin

Anti-Pyrin

品牌
Sigma-Aldrich
货号
ABF1169
规格纯度
from rabbit, purified by affinity chromatography
价格
1415.25 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Pyrin
Marenostrin
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Pyrin (UniProt: O15553; also known as Marenostrin) is encoded by the MEFV (also known as MEF, TRIM20) gene (Gene ID: 4210) in human. Pyrin is a homotrimeric protein that is involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. It organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Pyrin acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence it may prevent excessive IL-1B- and IL-18-mediated inflammation. Pyrin is expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes, but not in lymphocytes. It is also detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Its expression is also detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines. Three isoforms of Pyrin have been described that are produced by alternative splicing. Isoform 1 associates with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles. In pre-apoptotic cells, it co-localizes with PYCARD/ASC in large specks (inflammasome). Isoform 2 is shown to be localized to the nucleus.

特异性

This rabbit polyclonal antibody detects Pyrin in human cells. It targets an epitope within 15 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Pyrin, isoform 1.

应用

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Pyrin in human tonsil, human cerebral cortex, human skeletal muscle, and human pancreatic cancer tissues.

Anti-Pyrin, Cat. No. ABF1169, is a rabbit polyclonal antibody that detects human Pyrin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

质量

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Pyrin in 10 ?g of THP-1 cell lysate.

目标描述

~82 kDa observed; 86.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Affinity Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human
包装 antibody small pack of 25 μL
technique(s) immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI登记号 NP_000234
UniProt登记号 O15553
运输 ambient

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map