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位置: 首页> 品牌> Sigma-Aldrich> Anti-PTEN mono-ubiquitylated K13

Anti-PTEN mono-ubiquitylated K13

品牌
Sigma-Aldrich
货号
ABS533
规格纯度
from rabbit, purified by affinity chromatography
价格
5016.79 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-PTEN mono-ubiquitylated K13
Phosphatidylinositol 3, 4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, Phosphatase and tensin homologue deleted on chromosome 10
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (UniProt: P60484; also known as Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN) is encoded by the PTEN (also known as MMAC1, TEP1) gene (Gene ID: 5728) in human. PTEN belongs to the family of lipid phosphatases. It contains an N-terminal phosphatase catalytic domain, a C2-domain that binds to phospholipid membranes, and a C-terminal regulatory tail. It is a dual-specific phosphatase, which dephosphorylates both lipid and protein substrates. PTEN blocks the phosphatidylinositol 3-kinase/Akt signaling pathway by removing the phosphate in the 3-position on phosphoinositide-3, 4, 5-trisphosphate (PIP3). Dephosphorylation of PIP3 is an important factor in cell growth, proliferation, apoptosis, and survival. Dephosphorylation of PIP3 blocks Akt signaling that can lead to higher activity of pro-apoptotic molecules such as Bad and Caspase-9. PTEN is considered a major tumor suppressor and its mutations are reported in several human tumors. Over seventy different types of PTEN mutation have been recorded in patients with Cowden syndrome. PTEN -/- mice exhibit higher levels of 3 -phosphorylated phospholipids and die during embryogenesis due to the failure of developmental apoptosis. Lysine 13 is one of the conserved sites for ubiquitination and monoubiquitination is essential for the movement of PTEN from cytoplasm to the nucleus. NEDD4-1 is identified as E3 ligase that induces both mono- and poly-ubiquitination of PTEN.

特异性

This rabbit polyclonal antibody detects phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN monoubiquitinated on Lysine 13. Immunogen sequence is conserved, hence, it is expected to react with all 3 isoforms.

免疫原

KLH-conjugated linear peptide corresponding to 12 amino acids surrounding ubiquitinated Lysine 13 from the N-terminal region of human PTEN.

Epitope: N-terminus

应用

Research Category
Signaling
Peptide Inhibition Analysis: A 1:1500 dilution from a representative lot blocked PTEN mono-ubiquitinated K13 in HEK293 cell lysate.



Anti-PTEN mono-ubiquitylated K13, Cat. No. ABS533, is a rabbit polyclonal antibody that detects phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN and has been tested for use in Western Blotting and Peptide Inhibition Assay.

质量

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PTEN mono-ubiquitinated K13 in 10 ?g of HEK293 cell lysate.

目标描述

~65 kDa observed; 47.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human
包装 antibody small pack of 25 μL
technique(s) inhibition assay: suitable (peptide)
western blot: suitable
同位素/亚型 IgG
NCBI登记号 NP_000305.3
UniProt登记号 P60484
运输 ambient
Gene Information human ... PTEN(5728), TEP1(7011)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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