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位置: 首页> 品牌> Sigma-Aldrich> Anti-Pst1 antibody produced in rabbit

Anti-Pst1 antibody produced in rabbit

品牌
Sigma-Aldrich
货号
SAB3501217
规格纯度
affinity isolated antibody
价格
4802.96 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Pst1 antibody produced in rabbit
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Pst1 Antibody: Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein (Catalog No. 95-151) was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (Δoch1 Δmnn1 Δmnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.

免疫原

Pst1 antibody was raised against a synthetic peptide from the amino terminus of yeast Pst1.The immunogen is located within amino acids 80 - 130 of Pst1.

应用

Pst1 antibody can be used for detection of Pst1 by Western blot and ELISA at 1 μg/mL.

外形

Supplied in PBS containing 0.02% sodium azide.

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
偶联物 unconjugated
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
形式 liquid
species reactivity yeast
浓度 1 mg/mL
technique(s) ELISA: suitable
同位素/亚型 IgG
UniProt登记号 Q12355
运输 wet ice
储存温度 ?20℃
Gene Information Saccharomyces cerevisiae... PST1(851625)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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