半岛bd体育手机客户端 说明
一般描述
Pseudomonas aeruginosais a rod shaped, gram negative, monoflagellated, aerobic to facultative anaerobe bacteria which commonly inhabits soil and aqueous environments.1,2P.aeruginosais considered an opportunistic human pathogen mainly causing disease in immunocompromised patients. It is especially fatal in cystic fibrosis (CF) patients, but also presents a major problem in chronic wounds, burn wounds and infection of implanted biomaterials such as catheters.3P. aeruginosa is a major cause of nosocomial infections which affect more than 2 million
patients every year and are accounted for around 90,000 deaths annually.3
It forms highly resistant biofilms on human tissues such as the lungs of CF patients or medical surfaces. OnceP. aeruginosainfection is established it is extremely hard to eradicate.3
The genome ofP. aeruginosaencodes a vast arsenal of virulence factors. However, theP. aeruginosaisolated from chronic infections expresses less virulence factors in comparison to isolates from acute infections but more readily form biofilms.1,4-6
特异性
Anti-P. aeruginosa antibody recognizesP. aeruginosawhole dead bacteria and lysate, the antibody has no cross reactivity with whole dead bacteria or lysate ofProteus mirabilis,Porphyromonas gingivalis,E. coli,Shigella flexneri,Enterococcus faecalis,Akkermansia muciniphilaorSalmonella enterica.
应用
The antibody may be used in various immunochemical techniques including Immunoblotting and ELISA. Detection of theP. aeruginosabands by Immunoblotting is specifically inhibited by the immunogen.
生化/生理作用
P. aeruginosacells possess a single flagellum and type 4 pili that are important for adhesion to the host epithelial cells, motility and can also initiate an inflammatory response.1 After adhesion,P. aeruginosaactivates Type 3 secretion system (T3SS) and injects cytotoxins into the host cell.1 Moreover,P. aeruginosasecretes several proteases, which can degrade host complement, mucins, and disrupt tight junctions6-7,. In addition to lipases and phospholipases, that degrade lipids in host cell membranes6, and expresses Lipopolysaccharide (LPS), that is involved in inflammatory response and antibiotic resistance8.
Antibiotic resistance to many classes of antibiotics is a major challange inP. aeruginosatreatment.P. aeruginosapossesses several resistance mechanisms such as, low permeability of the outer membrane,
expression of membrane efflux (Mex) pumps, and ?-lactamase and AmpC that hydrolases ?-lactam antibiotics such as, penicillin9. In addition, as a result of genetic transfer new resistant strains emerge constantly. Therefore, finding new prevention and treatment strategies forP. aeruginosainfection is of high importance.1
外形
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
储存及稳定性
For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses,in vitrodiagnostic uses,ex vivoor in vivotherapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 形式 | liquid |
| species reactivity | Pseudomonas aeruginosa |
| 包装 | pkg of 100 μL pkg of 25 μL |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: 1:10,000-1:20,000 usingP.aeruginosalysate indirect ELISA: 1:5000- 1:0,000 using whole deadP.aeruginosabacteria for coating |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
Sigma-Aldrich
m.cnreagent.com
