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位置: 首页> 品牌> Sigma-Aldrich> Anti-Protein-arginine deiminase 4 Antibody, clone 10F1

Anti-Protein-arginine deiminase 4 Antibody, clone 10F1

品牌
Sigma-Aldrich
货号
MABE254
规格纯度
clone 10F1, from mouse
价格
5426.21 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Protein-arginine deiminase 4 Antibody, clone 10F1
HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Protein-arginine deiminase type-4 (PAD4; formerly PAD5) is one of five PAD (1-4, 6) homologues that belong to the superfamily of multidomain amidinotransferases. The PAD enzymes catalyze deiminating reactions in which guanidinium is hydrolyzed to peptidylcitrulline. PAD4 is a calcium-regulated nuclear-localized enzyme. It modifies arginine residues on histones H2A, H3, and H4 (H2 Arg3; H3 Arg2, H3 Arg8, H3 Arg17, H3 Arg26; and H4 Arg3). Several studies have suggested that PAD4-mediated citrullination has important implications for gene regulation. PAD4 may perform deimination at sites overlapping with arginine methylation, and antagonize the transcription-promoting effects of methylating enzymes such as CARM1 and PRMT1. PAD4 may therefore inhibit gene expression, most notably expression of cell-cycle regulating p53 genes such as p21. Previous studies have suggested that PAD4 may play central role in the development of cancer.

免疫原

GST-tagged recombinant protein corresponding to human Protein-arginine deminiase 4.

应用

Use Anti-Protein-arginine deiminase 4 Antibody, clone 10F1 (mouse monoclonal antibody) validated in WB, IP to detect PAD-4 also known as HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV.
Research Category
Epigenetics & Nuclear Function
Immunoprecipitation Analysis: A representative lot immunoprecipitated Protein-arginine deminiase 4 in IP (Prof. K. Mowen, Department of Chemical Physiology, The Scripps Research Institute-California Campus.).

Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Protein-arginine deminiase 4 in ChIP-Seq (Price, D. H., et al. (2011). Mol Cell. 45(1):38-50.).
Research Sub Category
Chromatin Biology

质量

Evaluated by Western Blot in DMSO treated and untreated HL-60 cell lysates.

Western Blot Analysis: 0.25 ?g/mL of this antibody detected Protein-arginine deminiase 4 in 10 ?g DMSO treated and untreated HL-60 cell lysates.

目标描述

~74 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
DMSO treated and untreated HL-60 cell lysates

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 10F1, monoclonal
species reactivity human
technique(s) ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_036519
UniProt登记号 Q9UM07
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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