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位置: 首页> 品牌> Sigma-Aldrich> Anti-PIKFYVE Antibody, clone R159.4.3C9

Anti-PIKFYVE Antibody, clone R159.4.3C9

品牌
Sigma-Aldrich
货号
MABS522
规格纯度
clone R159.4.3C9, from mouse
价格
5520.38 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-PIKFYVE Antibody, clone R159.4.3C9
1-phosphatidylinositol 3-phosphate 5-kinase, Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 2
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

1-phosphatidylinositol 3-phosphate 5-kinase (EC 2.7.1.150; UniProt Q9Y2I7; also known as Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 29) is encoded by the PIKFYVE (also known as CFD, FAB1, HEL37, KIAA0981, PIP5K, PIP5K3, ZFYVE29) gene (Gene ID 200576) in human. PIKfyve is a class III lipid kinase that is responsible for phosphorylating phosphatidylinositol (PtdIns) and phosphatidylinositol 3-phosphate (PtdIns3P) of their inositol ring at the D-5 position to form PtdIns3P and PtdIns(3,5)P2 (phosphatidylinositol 3,5-bisphosphate), respectively. PIKfyve forms a stable ternary heterooligomeric complex with its regulator ArPIKfyve (encoded by the VAC14 gene) and the Sac1 domain-containing PtdIns(3,5)P2 5-phosphatase Sac3 (encoded by the FIG4 gene), and both ArPIKfyve and Sac3 are required for maximal PIKfyve activity. PIKfyve is also known to interact with the Rab9 effector RABEPK and the kinesin adaptor JLP (encoded by SPAG9). By regulating endosomal trafficking, PIKfyve plays an important role in multiple biological processes, such as GLUT4 translocation, retroviral budding, autophagy, and neurodegeneration. Human PIKfyve is initially produced as a 2098-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. PIKFYVE gene mutation is linked to corneal fleck dystrophy (CFD) characterized by white flecks scattered in all levels of the stroma.

特异性

Expected to react with all four spliced isoforms of human PIKfyve reported by UniProt (Q9Y2I7-1 through Q9Y2I7-4).

免疫原

AviTag-6xHis-tagged recombinant protein corresponding an internal fragment of human PIKFYVE.
Epitope: Internal (N-terminal half).

应用

Research Sub Category
General Post-translation Modification
Research Category
Signaling
Anti-PIKFYVE Antibody, clone R159.4.3C9 is an antibody against PIKFYVE for use in Western Blotting.
Western Blotting Analysis: 1.0 ?g/mL from a representative lot detected PIKFYVE in 10 ?g of HepG2 cell lysate.

质量

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected PIKFYVE in 20 ?g of HL-60 cell lysate.

目标描述

~240/50 kDa observed. 237,1 kDa (isoform 1), 50.21 kDa (isoform 2), 51.48 kDa (isoform 3), 61.60 kDa (isoform 4) calculated.

外形

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 R159.4.3C9, monoclonal
species reactivity human
technique(s) western blot: suitable
同位素/亚型 IgG2aκ
NCBI登记号 NP_055855
UniProt登记号 Q9Y2I7
运输 wet ice
Gene Information human ... PIKFYVE(200576)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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