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位置: 首页> 品牌> Sigma-Aldrich> Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2

Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2

品牌
Sigma-Aldrich
货号
MAB1059
规格纯度
clone 10F12.2, Chemicon?, from mouse
价格
5688.25 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2
pigment epithelium-derived factor, proliferation-inducing protein 35, serine (or cysteine) proteinase inhibitor, clade F (alpha-2

antiplasmin, pigment epithelium derived factor), member 1, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink, 1991). More recently, PEDF was shown to have neurotrophic activity in the retina in vivo (Cayouette, 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson, 1999; Stellmach, 2001).

特异性

Recognizes Pigment-Epithelium Derived Factor (PEDF).

免疫原

Recombinant human GST-PEDF fusion protein. The sequence corresponding to amino
acids 196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. Coli. GST-PEDF is about 46 kDa on SDS-PAGE.

应用

Research Sub Category
Neurochemistry & Neurotrophins
Research Category
Neuroscience
Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2 is an antibody against Pigment Epithelium Derived Factor for use in ELISA, IC, IH & WB.
Western Blot:
Recognizes a protein with a molecular weight of ~46 kDa when tested against immunogen. Western blot positive control is available (Catalog number AG672)

ELISA:
A previous lot of this antibody was used on ELISA.

Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry.

Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.

Optimal working dilutions must be determined by end user.

质量

Routinely evaluated by Western Blot on human placenta lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected pigment epithelium on 10 μg of human placenta lysate.

目标描述

~46 kDa

外形

Purified mouse monoclonal IgG2a in buffer containing 0.02 M PBS, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
Format: Purified
Protein A purified

储存及稳定性

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

分析说明

Control
Eye tissue.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 10F12.2, monoclonal
species reactivity human
species reactivity (predicted by homology) rat
manufacturer/tradename Chemicon?
technique(s) ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型 IgG2a
NCBI登记号 NM_002615.4
UniProt登记号 P36955
运输 wet ice

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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