Anti-PI3 Kinase Antibody, p85 Antibody, N-SH3 Antibody, clone 1D5.1

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一般描述

Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: P27986; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the PIK3R1 (also known as GRB1) gene (Gene ID: 5295) in human. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. PI3 kinase alpha plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in PIK3R1 gene are known to be associated with insulin resistance.

特异性

Clone 1D5.1 detects p85 subunit of human and murine PI-3 kinase alpha. It targets an epitope within 77 amino acids from the N-terminal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 77 amino acids from the N-terminal region of the p85 regulatory subunit of human PI 3-kinase alpha.

应用

Western Blotting Analysis: 0.5 ?g/mL from a representative lot detected PI3 Kinase, p85, N-SH3 in 10 ?g of human cerebral cortex tissue lysate.

Anti-PI3 Kinase, p85, N-SH3, clone 1D5.1, Cat. No. MABS831, is a mouse monoclonal antibody that detects human and murine p85 subunit of PI3 kinase. It has been tested for use in Western Blotting.
Research Category
Signaling

质量

Evaluated by Western Blotting in NIH/3T3 L1 cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected PI3 Kinase, p85, N-SH3 in 10 ?g of NIH/3T3 L1 cell lysate.

目标描述

~85 kDa observed; 83.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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